Neuronal connections at different synapses form the complex activities of the brain. Therefore, developing technologies to dissect brain circuits has become imperative for neuroscience to understand brain functions. Genetically engineered recombinant viral vectors have become powerful tools for visualizing neural connectivity due to their ability to efficiently enter cells and deliver various genes. Creative Biogene offers a variety of RABV-derived neuronal tracing tools. These vectors have higher efficiency, specificity, and the ability to visualize complex neural connections across multiple synaptic steps. Our advanced neuronal tracing tools are designed to provide unparalleled insights into brain circuits, overcome the limitations of traditional tracers, and harness the power of recombinant viral vectors for precise cell-specific neuronal mapping.

At Creative Biogene, we understand the complexity of neuronal connectivity and its critical role in brain function. Our advanced neuronal tracing tools are designed to provide unparalleled insights into brain circuits, overcome the limitations of traditional tracers, and harness the power of recombinant viral vectors for precise cell-specific neuronal mapping. Creative Biogene offers a variety of PRV-derived neuronal tracing tools. These vectors have higher efficiency, specificity, and the ability to visualize complex neural connections across multiple synaptic steps.

Herpes simplex virus 1 (HSV-1) belongs to the α-subfamily of the Herpesviridae family and is distinguished from the β- and γ- subfamilies by its rapid reproduction rate and ability to infect and remain latent in neurons. As a ubiquitous and highly contagious human pathogen, HSV-1 has a very high infection rate worldwide. HSV infects epithelial cells in the skin, spreads through sensory nerves, and ultimately reaches the neuronal perikarya in the central nervous system. These findings inspired scientists to test the potential of HSV for neural circuit tracing in the 1980s.

Human Retinal Microvascular Endothelial Cells from Creative Bioarray are isolated from human retinal tissue. Human Retinal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Human Brain Microvascular Endothelial Cells from Creative Bioarray are isolated from human brain tissue. Human Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Human Testicular Endothelial Cells are isolated form normal human tesis tissue. T25 flasks is required for cell adhension to the culture vessels. Grow cells in ECM-coated culture vessels with 5% CO2. Each vial contains at least 1x10^6 cells per ml.

GPCR drug screening is essentially based on testing available molecule libraries. Such libraries can contain thousands of different compounds derived either by natural sampling, random synthesis or both, which are tested for their ability to bind to or interfere with the function of a GPCR molecule. Given the large number of molecules in those libraries, the use of high-throughput screening technologies that enable rapid testing through miniaturization and parallelization is indispensable. Creative Bioarray offers a comprehensive selection of binding and functional GPCR assays to meet the GPCR drug discovery research needs of both chemists and biologists. As a pioneer in this field, we can save your time and cost of the discovery programs by taking advantage of our expertise and innovative technology platforms.